Osmosis lab Essay

The Measurement of the Rate of Osmosis by using Deshelled volaille Eggs (Effect of Solute Concentration upon Rate/Degree of Osmosis in Chicken Eggs) accessEvery stall demand a mechanism that it uses in the maintenance of a constant internal environment. This is important in the control of the ever changing external environment to the cell. The transfer of materials to and from the cell thus needs a very stable mechanism to achieve this status. Cells ar accordingly bound a tissue layer that acts the selective controller of the incitement of diametrical substances to and from the cell. This is especially when dealing with the solutes both to and from the cell. Some solutes must be leave behinded to lean into and out of the cell depending on the need and urgency of the very solutes.This tissue layer is considered to selectively permeable to distinct solutes and thus pass on only al humbled the passage of specific solutes to and from the cell. This implies that the membrane to most cells is selectively permeable or has a assortedial permeability to different solutes. both(prenominal) the internal and the external environment to the cell be composed an aqueous base that is do of dissolved organic and inorganic substances.The gradual or spontaneous apparent motion of these substances in and out the cell are guided by a mechanism called diffusion. This is a movement by molecules to a region of sink minginess from that of higher minginess. A good number of studies swallow been utilise in the biology field that are related to the use and wideness of such passive movements in the cells and the entire organisms. There exist a number of passive movements that are vital to the functioning of several organisms. These acknowledge movements care diffusion, osmosis and early(a)s. However, the wallpaper allow for be pegged onto the factors related to osmosis. The prove will try to underscore the splendor of osmosis using the shelled ball cells. T his will similarly encompass the effects of several salt compactnesss to the egg cells.Research QuestionsThe paper tires to answer a number of questions related to osmosis. First, it seeks to understand and illustrate the magnificence of osmosis to the cellular life of an organism. It will also seek to underscore the brioof the osmosis put to work to the mode of transport in the egg cells. It will go ahead to explain the relevance of different rootages to the pelt cells. It then explains the component and importance of osmosis to the tissues and cells in the body.When dealing with osmosis, we use several terms. These are the hypertonic, hypotonic and the isotonic. These are used in relation to the relative parsimoniousnesss of such root words to the cell sap being investigated. Curtis defined these terms as illustrated below. Hypotonic is the outcome that contains a low concentration of the solutes particles. piss will of all time move away from the hypotonic firmness. In a typical cell, the hypotonic solution becomes the reference percentage point or the control in the essay fair(a) like in this experiment. This implies that the solution has lesser particles in comparison to that of intracellular space.The hypertonic solution on the another(prenominal) hand, refers to the solution that contains higher concentration of particles. It refers to the solution that has a higher concentration s compared to that of the intracellular space. Water will always move crosswise the trailer truck permeable membrane into the hypertonic solution in the cellular setting. This simply implies that the hypertonic solution has more concentration of the particles than the intracellular space. Finally, the isotonic solution contains the solutes with the same concentration as those of the other solution in comparison. When these two isotonic solutions are separated by a semi permeable membrane, then on that point will be a net flow of irrigate across the membranes .Research HypothesisIt is right to pre-empt that the bombard cells will react to the different solutions used in the experiment. This will be in the three different ways discussed above as hypotonic, hypertonic or isotonic solutions. The solutions will have a relative concentration in comparison to that of the eggs cells sap.Materials and MethodsPart I Effect of the Solute ConcentrationEach group of students were given(p) 2 chicken eggs from which the shell hadbeen dissolved away. The remaining membrane that was the shell membrane was considered to be differentially permeable. Each egg was false to have approximately the same concentration of solute in the membrane. Based on the rate of osmosis, the experiment attempted to determine what the concentration must have been. Each egg was then weighed separately to the nearest 0.1g and results recorded the weights in a Table at time 0.Each egg was once again placed into separate beakers containing solutions of either distilled water (0%), 10% saccharose, 20% sucrose, 30% sucrose, 40% sucrose and an unknown sucrose solution.At 15 refined intervals that is after 15, 30, 45, 60, and 75 minutes, the eggs were removed from the beakers. They were then carefully wiped complete all excess water and again each egg weighed separately. recruit the weights or masses were then recorded and weight changes placed in the Tables. Plot the changes in weight of each of the eggs were then plan against time in a graph as shown in the results section. some(prenominal) questions were then answered based on the results obtained. Which solutions would you say were hypotonic to that of the eggs? Which of them were hypertonic? Isotonic if there were any?What would you expect to happen if an egg was entrust into a sixth beaker containing a 50% sucrose solution? Results interpret 1 Weight of Eggs versus TimeGraph 2 reposition in weight of eggs versus TimeGraph 3 stretch out Minute versus Sucrose ConcentrationThe osmotic pressure in the cells and tissues usually take the same function and illustration as those in vivo tests. This shape is usually attained through the use of some controls in the experiment with known concentrations. The relativity of the solutions could then be detected using a very simple comparison exercise just like illustrated above. Both the vitro and the vivo tests take the same parameters in such experiments. This implies that the same results will be viewed if such experiments are carried out on mammalian cells like the shelled eggs cells used in this experiment.The fluid under test could be hypertonic, hypotonic or isotonic to the cell sap of the eggs cells used in this experiment in one way or the other. Just as illustrated in the introductory part of this paper, the hypotonic is the solution that contains a low concentration of the solutes particles. Water will always move away from the hypotonic solution. In a typical cell, the hypotonic solution becomes the reference point or the control in the experiment just like in this experiment. This implies that the solution has lesser particles in comparison to that of intracellular space.The hypertonic solution on the other hand, refers to the solution that contains higher concentration of particles. It refers to the solution that has a higher concentration s compared to that of the intracellular space. Water will always move across the semi permeable membrane into the hypertonic solution in the cellular setting. This simply implies that the hypertonic solution has more concentration of the particles than the intracellular space. Finally, the isotonic solution contains the solutes with the same concentration as those of the other solution in comparison. When these two isotonic solutions are separated by a semi permeable membrane, then there will be a net flow of water across the membranes.DiscussionFrom the results in the section above, it is important to underscore the vitality of the biological membranes in the musical arrangement of most organisms. It is clear that all biological membranes are indeed semi permeable to specific solutes. This implies that they are selectively permeable to water molecules and other important solutes in the functioning of such organisms. However, their great importance comes in when they are impermeable to several other solutes in the body that are neither important to the cells nor the tissues of the body. These solutes that are impermeable to the biological membranes include solutes like the charged molecules that include ions of Na, K, Ca and Cl.The isotonic solution used in this experiment contained 30 % sucrose and this acted as the control to the experiment. The concentrations below this concentration of the isotonic solution were considered as hypotonic andthese incl.ude the 10 and the 20 % sucrose solutions in the experiment. The other concentrations that were above this isotonic value gave the hypertonic solutions and this was the 40 % sucrose soluti on. Water molecules moved from the hypotonic solution to the inside of the eggs cells to make them swell and become larger and even a god number of these cells were burst due to the excess osmotic pressure built by the effect of the hypotonic solution.The eggs cells that were placed in the isotonic solution never gave any visible change in their mold and structure as the concentrations for the solution and that of the cell sap were similar and equal. Those eggs cells that were placed in the hypertonic solution became flaccid. This was due to the movement of water molecules out of the cell into the hypertonic solution. Indeed, the solution in this case change magnitude in volume as opposed to the hypotonic solution that trim down in volume.ConclusionIn a nut shell, the experiment prove successful as the objectives of the practical were met to the letter. The hypothesis also proved effectual and in line with the objectives. It implies that the different concentrations of sucros e used in the experiment gave varied effects to the cell sap of the eggs cells. The practical understandably demonstrated the effects and process of osmosis to the eggs cells. It also demonstrated the importance of different solutions to the functioning of the cells and tissues in the body of mammals. This was in response to the process of osmosis in the body.